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. 2020 Sep 10;20(5):223. doi: 10.3892/ol.2020.12086

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Copyright: © Shen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Downregulation of DKK3 counteracted the proliferation arrest by miR-3690-in. (A) Western blot analysis verified that silencing DKK3 effectively decreased the expression of DKK3 in miR-3690-in-transfected K1 cells. (B) miR-3690-in-transfected K1 cells after transfection with DKK3-siRNAs promoted the anchorage-independent growth. Representative quantification of colonies that were >0.1 mm. (C) Representative quantification of the BrdU incorporation assay. Each bar represents the mean of three independent experiments. *P<0.05. miR-3690-in, microRNA-3690-inhibitor; DKK3, Dickkopf-related protein 3; siRNA, small interfering-RNA; NC, negative control.