Fig. 2.

Overexpression of linc00514 in breast cancer cells promotes M2 polarization of macrophages. a-b. Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) were transiently transfected with linc00514 siRNAs (Si-linc00514) or linc00514 plasmids (pcDNA-linc00514) for 48 h before the co-culture. After co-culturing breast cancer cells with PMA-induced human monocyte THP-1 cells for 48 h using Transwell assay, we detected the relative mRNA levels of CD206 and CD163 in THP-1 derived macrophages, both of which were M2 polarization markers of macrophages. c. The percentage of CD206+ or CD163+ cells after overexpressing linc00514 was detected using flow cytometry. d. Female Balb/c nude mice were subcutaneously injected with the MDA-MB-468 cells (5 × 10⁶ cells) which were stably transfected with linc00514-overexpressing plasmids (linc00514-OVE) or the control plasmids (n = 5 in each group). After 7 weeks, the expression of F4/80 and CD206 in tumor tissues was detected using immunohistochemistry. Scale bar = 120 μm. Three independent experiments. ** P < 0.01 vs control (RPMI). ## P < 0.01 vs control (Si-ctrl or pcDNA)