Table 2.
qRT-PCR Primers (from 5′ to 3′) | |
---|---|
Linc00514 | F: GTGTGCCTGCCCTTTCCTGTG |
R: TCCCATCCCATTCCCATCACCAG | |
CD206 | F: TTCAGTGGACCATCGAGGAAGAGG |
R: ATGGCAACACACCCTGGCTTTC | |
CD163 | F: GCCACAACAGGTCGCTCATCC |
R: GCAAGCCGCTGTCTCTGTCTTC | |
Jag1 | F: TGTGGCTTGGATCTGTTGCTTGG |
R: ACGTTGTTGGTGGTGTTGTCCTC | |
Hes1 | F: GGCTGGAGAGGCGGCTAAGG |
R: TGCTGGTGTAGACGGGGATGAC | |
GAPDH | F: CAATGACCCCTTCATTGACC |
R: GACAAGCTTCCCGTTCTCAG | |
Si-RNA sequences | |
Si-control | UUCUCCGAACGUGUCACGUCU |
Si-linc00514 | GGACCACACUGGGAUUCUAGA |
Si-STAT3 | GAGUCAAGGAGACAUGCAAGA |
Si-jag1 | GGGUCAGAAUUGUGACAUAAA |
Si-jak2 | GAUGGAUAUUAUAGAUUAACU |
Agarose gel electrophoresis primers | |
Linc00514 | F: CAGGACCTTCGCAGGTGCTG |
R: CTTCTGGGGTTTTCCACCCCC | |
RNA Pull down Probe | |
CATCCCTTCCTCTCTGCTCAGGACCAGGGCCAGGCCCCTACATCAGTAACCCCCAACCCCTAAGACCTCCATGCTCCCAGAACTCCAGGGCCTTACCTGCCTGGGTTCCAGCTCCCAGGAAGGCCCCGACTCCCCACCCATTCCTATTCCCAAATCCCAGGGGACAGAGCCAAAGAG | |
FISH Probe | |
GCCATGTGGAGTGCGGGTCCGTGGTCCAAACACCACCCAGGGCCACTGTGCTCCACACCCTGACAGTTCACTAAGGGCGCATGCCCAGGAAGCAGCTCAGAGGCCTCCACAGGAGTCCCCACCCACAGGAATGGCATTGGCAGGCCACAGAGGGACGAGTGCCTGGGGAGGGTAACACAGACCGAGAGGGGGACAGAGAGGGACAGGACATGGTGAGTGTGGCATGGAGGTGGCACACGGGCGGGGTCCTCCCTTCATCCGCTTCTGAGGTTCCCTCTTCCCCAGGCCTGGGGAGAGCTAGAAAGAGGCTGACATTGACCCTAAGAGCTCCTCCCCTAAGAATGAGGGTTGGGACCCTAAACCCTCCTGCCCGCCCCTCCCTGCGGCCCCTGCCCAGCCACCCTCACCTG |
Abbreviation: si-RNA Small interfering RNA; FISH Fluorescence in situ hybridization