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. 2020 Sep 17;39:191. doi: 10.1186/s13046-020-01676-x

Table 2.

The primers, small interfering RNA sequences, and probes used in this study

qRT-PCR Primers (from 5′ to 3′)
 Linc00514 F: GTGTGCCTGCCCTTTCCTGTG
R: TCCCATCCCATTCCCATCACCAG
 CD206 F: TTCAGTGGACCATCGAGGAAGAGG
R: ATGGCAACACACCCTGGCTTTC
 CD163 F: GCCACAACAGGTCGCTCATCC
R: GCAAGCCGCTGTCTCTGTCTTC
 Jag1 F: TGTGGCTTGGATCTGTTGCTTGG
R: ACGTTGTTGGTGGTGTTGTCCTC
 Hes1 F: GGCTGGAGAGGCGGCTAAGG
R: TGCTGGTGTAGACGGGGATGAC
 GAPDH F: CAATGACCCCTTCATTGACC
R: GACAAGCTTCCCGTTCTCAG
Si-RNA sequences
 Si-control UUCUCCGAACGUGUCACGUCU
 Si-linc00514 GGACCACACUGGGAUUCUAGA
 Si-STAT3 GAGUCAAGGAGACAUGCAAGA
 Si-jag1 GGGUCAGAAUUGUGACAUAAA
 Si-jak2 GAUGGAUAUUAUAGAUUAACU
Agarose gel electrophoresis primers
 Linc00514 F: CAGGACCTTCGCAGGTGCTG
R: CTTCTGGGGTTTTCCACCCCC
RNA Pull down Probe
 CATCCCTTCCTCTCTGCTCAGGACCAGGGCCAGGCCCCTACATCAGTAACCCCCAACCCCTAAGACCTCCATGCTCCCAGAACTCCAGGGCCTTACCTGCCTGGGTTCCAGCTCCCAGGAAGGCCCCGACTCCCCACCCATTCCTATTCCCAAATCCCAGGGGACAGAGCCAAAGAG
FISH Probe
 GCCATGTGGAGTGCGGGTCCGTGGTCCAAACACCACCCAGGGCCACTGTGCTCCACACCCTGACAGTTCACTAAGGGCGCATGCCCAGGAAGCAGCTCAGAGGCCTCCACAGGAGTCCCCACCCACAGGAATGGCATTGGCAGGCCACAGAGGGACGAGTGCCTGGGGAGGGTAACACAGACCGAGAGGGGGACAGAGAGGGACAGGACATGGTGAGTGTGGCATGGAGGTGGCACACGGGCGGGGTCCTCCCTTCATCCGCTTCTGAGGTTCCCTCTTCCCCAGGCCTGGGGAGAGCTAGAAAGAGGCTGACATTGACCCTAAGAGCTCCTCCCCTAAGAATGAGGGTTGGGACCCTAAACCCTCCTGCCCGCCCCTCCCTGCGGCCCCTGCCCAGCCACCCTCACCTG

Abbreviation: si-RNA Small interfering RNA; FISH Fluorescence in situ hybridization