Figure 4.

Relative expression of VEGF/CCR-7 mRNA and protein following transfection with miR-130a, miR-25 and miR-191* mimics and inhibitors. (A) The relative expression of VEGF and CCR-7 mRNA in A549 cells transfected with miR-130a, miR-25 and miR-191* mimics and inhibitors. The dotted line represents the level of the control group. (B) The relative expression of VEGF and CCR-7 protein in A549 cells transfected with miR-130a and miR-25 mimics. Western blotting and reverse transcription-quantitative PCR were performed in triplicate, and each experiment was repeated ≥3 times. *P<0.05 and **P<0.01 vs. control. VEGF, vascular endothelial growth factor; miR, microRNA; CCR-7, C-C chemokine receptor type 7; MC, mimics control.