Fig. 1.
Illustration of a 3D bioprinting nozzle, showing cell-laden O2-releasing bioink and the chemical reaction leading to the formation of O2. B) Optical image of a 3D bioprinted construct with 0.5 CPO. C) Optical illustration of cardiomyocyte-laden printed construct cultured for 7 days and ready for delivery by injection (orifice diameter: 2.5mm). D) Image of printed construct shown in C after multiple suction and release by injection, which demonstrated its mechanical stability. E) Live/dead staining florescent microscopic image of the printed construct at day 7 after incubation and after injection, demonstrating that cardiomyocytes can survive in the construct even after multiple injection process (Green shows live cells and red shows dead cells) (Scale bars for (B), (C), (D): 2mm; (E): 200 μm).