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. 2020 Sep 11;20(5):227. doi: 10.3892/ol.2020.12090

Figure 3.

Figure 3.

DNA damage repair in A549/DDP cells is regulated by BCLAF1. (A) Representative immunofluorescence images of γH2AX and BCLAF1 in A549/WT and A549/DDP cells transfected with siBCLAF1 or siCTRL. Cell lines were treated with 4 µM cisplatin for 10 h. Magnification, ×600. (B) Quantification of γH2AX and BCLAF1 fluorescence intensity in A549/WT and A549/DDP cells transfected with siBCLAF1 or siCTRL. (C) The protein expression levels of γH2AX and BCLAF1 were measured by western blotting in A549/WT and A549/DDP cells transfected with siBCLAF1 or siCTRL. Cells were treated with 4 µM cisplatin for 10 h. (D) Quantification of γH2AX and BCLAF1 protein expression in A549/WT and A549/DDP cells transfected with siBCLAF1 or siCTRL. (E) The expression levels of BCLAF1 was measured in A549/DDP cells by western blotting. Cells were transfected with either control or BCLAF1 siRNA for 48 h. Data are presented as the means ± SD from three independent experimental repeats. *P<0.05, **P<0.01. DDP, cisplatin resistance; WT, wild-type; BCLAF1, bcl-2-associated transcription factor 1; si, small interfering; CTRL, control; γH2AX, γH2A histone family member X.