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. Author manuscript; available in PMC: 2020 Sep 18.
Published in final edited form as: Pediatr Res. 2015 May 7;78(2):128–136. doi: 10.1038/pr.2015.88

Figure 3.

Figure 3.

MKP-1 knockdown in rIEC-6 cells. (a) rIEC-6 cells were transiently transfected with either siRNA against MKP-1 (siMKP-1; black bars) or scramble siRNA (scramble; white bars). Twenty-four hours after transfection, the cells were stimulated with LPS (100 μg/ml) for the indicated times. MKP-1 expression was evaluated by qPCR following transfection; *P < 0.05 vs. scramble for each time point. (b) Representative western blot of MKP-1 protein expression after transfection with siRNA for MKP-1 or scramble siRNA. Following transfection, rIEC cells were stimulated with LPS for 24 and 48 h. (c) Densitometry data shown as means ± SEM of protein level relative to β-actin protein levels for triplicate samples from three independent experiments. *Protein expression of MKP-1 after siMKP-1 transfection and LPS stimulation for 48 h is different from MKP-1 protein expression after scramble transfection and LPS stimulation for 48 h, P < 0.05.