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. 2020 Sep 4;8:578005. doi: 10.3389/fbioe.2020.578005

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Copyright © 2020 Song, Wu, Wang, Wang, Chai, Ding, Li, Yao, Xiao and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of precursor engineering on crocetin output in S. cerevisiae. (A) The paradigm of crocetin biosynthetic pathway optimization by precursor engineering in S. cerevisiae; (B) The relative acetyl-CoA concentration per gDCW in the gene knockout (ΔCIT2 and ΔMLS) strains; (C) The effects of gene knockout on crocetin and zeaxanthin output. The production of crocetin and zeaxanthin was quantified by μg/gDCW. The error bars represent standard deviations calculated from triplicate experiments.