Fig. 4. In vitro therapeutic performance of COF-survivin. (A) Cell viabilities of MCF-7 cells treated with different concentrations of COF-survivin followed by 5/10 min laser irradiation. (B) Live/dead cell staining assay, Scale bar = 100 nm. (C) Detection of intracellular ROS with DCFH-DA, scale bar = 250 nm. (D) Evaluation of ΔΨm with Rhodamine 123, scale bar = 250 nm. (E) Immunofluorescence staining of intracellular caspase-3 expression with different treatments, scale bar = 250 nm. (F) Flow cytometry analysis of cell apoptosis following different treatments. The concentration of COF-survivin for cancer cell treatment was 100 μg mL–1, the laser power was 0.2 mW cm–2, and irradiation duration was 10 min. I: PBS only, II: PBS + laser, III: COF-survivin, IV: COF-survivin + laser (***p < 0.001).
