Figure 2.

3T Magnetic Resonance Imaging. Piglets were scanned at 24 and 48 h in the Philips Achieva clinical magnet. ¹H- Magnetic Resonance Spectroscopy (MRS) (A) was acquired using chemical shift imaging (CSI) with 8 × 8 matrix and 8 × 8 × 10 mm³ voxels, TR/TE was 2,000 ms/288 ms. The spectral width was 2 kHz with 2,048 points. Voxels selected were over the left thalamus [basal ganglia, thalamus (BGT)] and left subcortical white matter (WM) at the level of the centrum semiovale. Spectra were processed with Tarquin included in basis set, and the ratio of lactate (Lac)/N-acetylaspartate (NAA) was calculated from the amplitude of the fitted components (Lac+Thr/NAA+NAAG). For diffusion-weighted imaging (DWI) (B), regional of interest (ROI) for mean diffusivity (MD) and fractional anisotropy (FA) in the deep gray matter (DGM) (red) and WM (green) were automatically identified by atlas label propagation. The internal capsule was selected to represent WM, and the caudate, putamen, globus pallidus, thalamus, and hypothalamus were used for DGM.