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. 2020 Sep 4;11:1934. doi: 10.3389/fimmu.2020.01934

Figure 2.

Figure 2

M2-exos protect EAE mice. (A) Clinical score of EAE mice treated with PBS, M2-CM, or M2-exo; (B) Observation of the morphology and size of M2-exos by TEM; (C) Exosome concentration and particle size by Nanosight Tracking Analysis; (D) Detection of exosome markers CD63, CD81, and TSG101 by western blot analysis; (E) Detection of demyelination in spinal cord sections using LFB staining; (F) Pathological examination of spinal cord sections in mice detected by HE staining; (G) T cell infiltration into the spinal cord of EAE mice detected with flow cytometry. Compared with the PBS group, **p < 0.01, ***p < 0.001. Data are analyzed with two-way ANOVA, and Tukey's multiple comparisons test was utilized for post hoc test n = 6.