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. 2020 Sep 4;11:1934. doi: 10.3389/fimmu.2020.01934

Table 1.

Primer sequences of RT-qPCR.

Gene Primer sequence
miR-21-5p F: 5′-TAGCTTATCAGACTGATGTTGA-3′
R: 5′-TCAACATCAGTCTGATAAGCTA-3′
GAPDH F: 5′-GGGAGCCAAAAGGGTCAT-3′
R: 5′-GAGTCCTTCCACGATACCAA-3′
U6 F: 5′-CGCTTCGGCAGCACATATAC-3′
R: 5′-AATATGGAACGCTTCACGA-3′
CCL22 F: 5′-ATTACGTCCGTTACCGTCTG-3′
R: 5′-TAGGCTCTTCATTGGCTCAG-3′
PPARγ F: 5′-GCCTTGCAGTGGGGATGT-3′
R: 5′-CTCGCCTTTGCTTTGGTC-3′
PVT1 F: 5′-GATGCCCCTCAAGATGGCTG-3′
R: 5′-GACACGAGGCCGGCCACGC-3′
SOCS5 F: 5′-ATAAAGTGGGAAAAATGTGG-3′
R: 5′-TTGCCTTGACTGGTTCTCGT-3′

RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR, microRNA; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PPARγ, peroxisome proliferator-activated receptor gamma; PVT1, plasmacytoma variant translocation 1; SOCS5, suppressor of cytokine signaling 5; F, forward; R, reverse.