Figure 6.

Densitometric studies of nitrotyrosine levels in Cav1^ΔEC mice. (A) Immunoblots staining for nitrotyrosine (3-NT) in protein lysates extracted from iridocorneal angles of WT and Cav1^ΔEC mice. Each column represents protein extracted from a single mouse (n = 3 for each group). There are four main bands evident. All signal intensities were normalized to glyceraldehyde-3-phosphate dehydrogenase. (B) Densitometric analysis depicting data for each separate band within each sample. There was a significant increase in tyrosine nitration of the Cav1^ΔEC compared with the WT samples for band 1 (P = 0.015) and band 2 (P = 0.019). There was no significant difference between Cav1^ΔEC and WT for bands 3 and 4. (C) Densitometric analysis depicting combined data for all four bands within each lane, for each group. When calculating total nitrotyrosine levels, there was no significant difference between WT and Cav1^ΔEC. All data were analyzed using unpaired t test, n = 3 for all groups. All densitometry data was normalized to WT control.