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Functions of corneal endothelial cells after siCOL8A2 transfection. (A) COL8A2 mRNA expression in rat corneal endothelium after COL8A2 siRNA transfection in vivo was reduced (4.73% ± 1.54%). Fold change means relative mRNA expression to control. (B and C) Corneal opacity during the experiments. (D) Alizarin S red staining showed CEC elongation, which was similar with the change in cultured cell shape after siCOL8A2 transfection. Bar: 100 µm. *Statistically significant.
