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. 2020 Sep 4;11:1313. doi: 10.3389/fpls.2020.01313

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Copyright © 2020 Berger, Boscari, Horta Araújo, Maucourt, Hanchi, Bernillon, Rolin, Puppo and Brouquisse

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histochemical localization of MtNRs expression in Medicago truncatula roots. Localization of GUS activity in transgenic M. truncatula roots expressing the gusA reporter gene under the control of a 1.65 Kb MtNR1 promoter fragment (A, B), of a 1.7 kb MtNR2 promoter (C, D) and of a 1.55 kb pMtNR3 promoter (E, F). Whole root segment 4 dpi with S. meliloti (A, C, E) and longitudinal section of a 2 wpi old nodules (B, D, F) were stained for 3, 5, or 16 h with X-gluc for the GUS activity for pMtNR2, pMtNR1, or pMtNR3 respectively. Zones I, II, and III of the nodule are represented in red in picture F. p, nodule primordium; Vb, vascular bundles; c, cortex. Scale bars, 50 µm for (A, C, E); 100 µm for (B, D, F).