Table 2.
Effects of NR effectors on ATP/ADP ratio in Medicago truncatula roots and nodules.
| ATP/ADP ratio | ||||
|---|---|---|---|---|
| 10 hpi | 4 dpi | 4 wpi | ||
| Control | 6.1 ± 1.1 | 6.3 ± 1.7 | 6.8 ± 1.2 | |
| +1 mM NO2− | 5.7 ± 0.9 | 4.7 ± 0.4 | 6.4 ± 0.7 | |
| +1 mM Tg | 5.2 ± 0.5 | 5.6 ± 3.3 | 4.4 ± 0.4 * | |
| +1 mM NO2− +1 mM Tg | 4.9 ± 0.6 | 4.5 ± 1.0 | 5.5 ± 0.7 | |
Either 10 hpi roots, 4 dpi roots, or 4 wpi-old nodules were incubated for 4 h in the presence of either 1 mM nitrite (NO2−), 1 mM tungstate (Tg), or both. Data are the means ± SE of three (10 hpi, 4 dpi) and four (4 wpi) biological replicates. Each measure was realized in three technical replicates. Asterisk * indicates statistical difference with the control at the same time point at P < 0.05 according to Student’s t test. dpi, day post inoculation; hpi, hour post inoculation; wpi, week post inoculation.