Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 17;8(2):e000638. doi: 10.1136/jitc-2020-000638

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Increased extracellular cytokine levels of TPC-1-induced macrophages are not caused by an enhanced cytokine secretion. Monocytes were cocultured with TPC-1 cells or monocytes for 24 hours in a transwell system. After removal of the transwell, (tumor-induced) macrophages were stimulated with LPS for 4 hours. On 1 hour with LPS, secretion was blocked with Brefeldin A. (A) Western blot analysis was performed to detect intracellular levels of TNF-alpha and β-actin as control. Quantification of TNF-alpha was performed relative to β-actin (right panel). (B) Extracellular TNF-alpha levels were determined by ELISA (n=2 donors). LPS, lipopolysaccharide; TNF-alpha, tumor necrosis factor alpha.