Table 2.

Effect of MLF on the physiological, serum biochemical, and antioxidant parameters of buffalo.

Parameter	Control	MLF15	MLF30	MLF45	SEM	P-Value
						Treatment	Linear	Quadratic
Physiological parameters
Body surface temperature (°C)	33.75	33.85	33.82	34.15	0.538	0.956	0.642	0.833
Rectal temperature (°C)	38.31b	38.50a	38.36ab	38.47a	0.049	0.047	0.142	0.477
Breathing frequency (times/ min)	21.22	20.33	21.15	21.06	0.732	0.812	0.921	0.592
Serum biochemical parameters
Total protein (g/L)	79.98	80.12	77.86	78.08	2.063	0.839	0.956	0.328
Albumin (g/L)	37.12	37.52	35.38	37.3	1.219	0.570	0.340	0.433
Globulin (g/L)	42.86	42.6	42.48	40.78	1.817	0.867	0.560	0.553
Blood urea nitrogen (mmol/L)	12.9	14.64	16.12	16.54	1.127	0.16	0.923	0.037
Glucose (mmol/L)	4.02	3.9	3.89	3.75	0.176	0.905	0.708	0.431
Insulin (ng/ml)	0.78b	0.99b	1.01b	1.26a	0.071	0.011	0.002	0.769
Serum antioxidant parameters
SOD (U/mL)	90.92	83.53	87.31	85.83	4.404	0.065	0.576	0.521
CAT (U/mL)	7.47a	5.36b	5.58b	5.29b	0.438	0.001	0.012	0.072
T-AOC (U/ml)	3.68a	2.07b	1.85b	2.24b	0.186	0.001	0.001	0.001
MDA (nmol/ml)	5.60a	3.26b	4.15ab	1.39c	0.467	0.012	p < 0.001	0.665
GSH-Px (U/ml)	639.67a	740.94b	788.71b	509.30c	26.177	p < 0.001	0.019	p < 0.001

^{a, b, c}Values with different superscripts in the same row differ significantly (p < 0.05).