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Mixed flavored e-liquid induces cytotoxicity in HFL-1. Total live cell counts of a (A) mixed flavored e-liquid, (B) nicotine or PG/VG controls in HFL-1 after 24 h exposure. Cells were stained with AO/PI dye and counted on a Cellometer Auto 2000. Data represented as mean ± SEM (n = 4 per group). *p ≤ 0.05, ***p ≤ 0.001 vs untreated control group. Only significant differences vs control were labeled with asterisks.
