Fig. 9.
The influence of three dystrophy-causing mutations on the intracellular pH (pHi) dependence of Slc4a11 at extracellular pH (pHe) 8.50. Three mutants (E369K, T370K, and R774H: equivalent to human dystrophy-causing mutants E399K, T401K, and R804H) were tested for their response to changes in pHi. A–C: a sampling of current-voltage (I-V) plots gathered from representative mutant-Slc4a11-expressing cells during experiments equivalent to those shown in Fig. 2, A, D, E, and F: The relationship between pHi and membrane conductance (Gm) for a greater number of mutant-Slc4a11-expressing oocytes drawn from all of the I-V plots gathered for each cell. Gray dashed line shows maximum Gm calculated from any of a greater number of water-control oocytes (from Supplemental Fig. 1B: https://doi.org/10.6084/m9.figshare.12400946). G–I: each Slc4a11 trace from D–F is shown here normalized to its own maximum conductance. Vm, membrane potential.