Fig. 3.

CFTR contributes to TNFα+IL-17-induced airway surface liquid (ASL) alkalinization. A: after TNFα+IL-17 treatment for 24 h, human airway epithelia were treated for 2 h with either apical vehicle (DMSO) or CFTR(inh)-172 suspended in a volatile solvent (Fluorinert FC-72) to achieve an approximate ASL concentration of 10 μM. pH of ASL (pH_ASL) was measured with SNARF-1-dextran in the presence of ${\text{HCO}}_3^{-}$/CO₂ (n = 6). B and C: effect of negative control (NC) or siRNA directed against CFTR on pH_ASL in vehicle- or TNFα+IL-17-treated epithelia (n = 5). Each data point represents epithelium from a different donor. Bars indicate means ± SD. Data were analyzed using repeated measures ANOVA with Tukey’s multiple comparisons test (A) or paired Student’s t test (B and C). *P < 0.05, **P < 0.01.