Figure 8. BCL-2 and BAX expression in HT-29, MCF7, and PC3 cancer cells.

In total, 10⁶ cells/well were seeded in 6-well plates and treated with PACs at their respective IC₅₀ concentration for 24 h. ΔΔ Ct method was used to calculate the mRNA expression level between treated cells and control. The Y-axis represents the fold of mRNA expression level. The data were analyzed using Student’s t-tests compared to control cells. The mean ±  SD of three biological replicates is plotted. (A, B) HT29, (C, D) MCF-7, and (E, F) PC-3, ns: p > 0.05, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control.