Figure 9. Caspase enzyme activity in HT-29, MCF7, and PC3 cancer cells.

In total, 10⁶ cells/well were seeded in 6 well plates and incubated for 24 h. Then, cells were treated with PACs at their respective IC₅₀ concentrations for 24 h. The obtained data were analyzed using Student’s t-tests compared to control cells. The mean ±  SD of three biological replicates is plotted (A–C) HT29, (D–F) MCF-7, and (G-I) PC-3. ns: p > 0.05, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 vs. control.