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| Primer | Sequence (5′–3′) | Tm (°C) |
Length (bp) |
Amplicon size (bp) |
|---|---|---|---|---|
| Bax _F | CGAGCAGATCATGAAGACCG | 52.9 | 20 | 300 |
| Bax _R | AAGTAGAACAGGGCCACCAC | 53.7 | 20 | |
| Bcl-2 _F | AGTACATCCACTACAAGCTGAG | 51.4 | 22 | 274 |
| Bcl-2 _R | TACCTCCTGCTGAAGTCGTC | 53.0 | 20 | |
| ACTB _F | TCCACGAGACCACCTTCAAC | 53.8 | 20 | 266 |
| ACTB _R | GTACTCCTGCTTGCTGATCC | 52.3 | 20 |
