Table 4.
Items | Supplemented Se level from Se yeast, mg/kg | SEM | P-values | ||||||
---|---|---|---|---|---|---|---|---|---|
0 | 0.05 | 0.15 | 0.25 | Treatment | Linear | Quadratic | Cubic | ||
TP, mg/ml | 5.54 | 5.59 | 5.20 | 5.22 | 0.176 | 0.424 | 0.177 | 0.755 | 0.458 |
ALB, mg/ml | 1.54 | 1.50 | 1.37 | 1.34 | 0.163 | 0.846 | 0.433 | 0.857 | 0.917 |
GLB, mg/ml | 4.00 | 4.10 | 3.83 | 3.88 | 0.278 | 0.941 | 0.675 | 0.926 | 0.679 |
ALB/GLB ratio | 0.407 | 0.378 | 0.427 | 0.350 | 0.074 | 0.991 | 0.845 | 0.855 | 0.964 |
ALT, U/L | 3.07 | 3.13 | 3.02 | 3.17 | 0.303 | 0.993 | 0.911 | 0.863 | 0.819 |
AST, U/L | 2.73 | 2.75 | 2.36 | 2.05 | 0.347 | 0.576 | 0.175 | 0.863 | 0.813 |
GSH-Px, mmol/ml | 173c | 274b | 365a | 272b | 6.5 | <0.001 | <0.001 | <0.001 | 0.393 |
SOD, U/ml | 22.2 | 22.8 | 25.1 | 26.3 | 4.61 | 0.910 | 0.478 | 0.956 | 0.939 |
CAT, U/ml | 1.71c | 2.51bc | 3.28ab | 3.90a | 0.376 | 0.003 | <0.001 | 0.470 | 0.712 |
TAC, U/ml | 3.53c | 5.65b | 10.6a | 5.61b | 0.251 | <0.001 | <0.001 | <0.001 | <0.001 |
MDA, nmol/ml | 32.8a | 23.4b | 18.8c | 22.5b | 0.38 | <0.001 | <0.001 | <0.001 | <0.001 |
MDA/TAC ratio | 9.55a | 4.16b | 1.78b | 4.03c | 0.373 | <0.001 | <0.001 | <0.001 | 0.0152 |
Se yeast, selenium-enriched yeast; SEM, standard error of the mean; TP, total protein; Alb, albumin; Glb, globulin; ALT, alanine transaminase enzyme; AST, aspartate transaminase enzyme; GSH-Px, glutathione peroxidase; SOD, superoxide dismutase; CAT, catalase; TAC, total antioxidant capability; MDA, malondialdehyde.
Means were calculated using six replicates per treatment (one bird per replicate).
Mean values within a row with different letters were significantly different (p < 0.05).