Figure 5.

Effect of PGE₃, 12-S-HEPE, and LTB₅ on Caco-2 cell growth. Caco-2 cells were incubated for 48 h with PGE₃ (0.1, 1, and 10 nM, line, dot, and gray bars, respectively) or PGE₃ (10 nM) plus SC19220 (SC, 60 nM) or AH 23848 (AH, 20 nM) or ONO-AE3-240 (ON, 2 nM) or with 12-S-HEPE (10, 100, and 1000 nM, line, gray, and dot bars, respectively) or with 12-S-HEPE (100 nM, gray bar) plus U 75302 (U, 5 µM) or LY 255283 (LY, 25 µM) or ketoprofen (Kp, 5 µM). Cell cultures were also incubated with LTB₅ (1, 10,and 100 nM, dot, gray, and black bars, respectively) or LTB₄ (10 nM, gray bar) in absence of FBS. Finally, cells were then counted (A, C, E) and DNA synthesis was measured (B, D). Data are expressed as means ± SEM of three to four experiments performed in triplicate. *P < 0.05 vs. Caco-2 cell cultures in the absence of FBS, ^#P < 0.05 vs. cells incubated with 10 nM PGE₃ or 12-S-HEPE.