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Effect of 10-HODE on Caco-2 cell growth and DNA synthesis. 10-HODE was incubated with Caco-2 cells without FBS in presence of U75302 (U75, 5 µM), LY25283 (LY25, 25 µM), or MK571 (MK5, 25 µM) for 48 h and then cells were counted or DNA synthesis assayed by cell BrdU incorporation. Results are expressed as mean ± SEM (n = 6–9) *P < 0.05 vs control group (cells cultured without FBS), #P < 0.05 vs 10-HODE (100 nM), N.D., non determined.
