Figure 6.

Inhibition of ROS accumulation and p38 activation suppressed apoptosis induced by TEOA. (A) OCI-LY10 cells were exposed to TEOA for 12 h with or without NAC (5 mM) pretreatment for 1 h, the levels of p-p38, total p38, and γ-H2AX were determined by western blot. The expression of p-p38 and γ-H2AX was quantified by Image J software. Data were presented as mean ± SD, **P<0.01. (B) OCI-LY10 cells were exposed to TEOA for 12 h with or without SB203580 (10 μM) incubation in advance for 1 h, the levels of p-p38, total p38, and γ-H2AX were determined by western blot. Corresponding expression analysis of p-p38 and γ-H2AX were shown on the right. Data are presented as mean ± SD, **P<0.01. (C) OCI-LY10 cells were treated with 25 µM TEOA for 12 h with or without SB203580 (10 μM) pretreatment for 1 h and stained with PI or γ-H2AX (1:200) primary antibody and FITC-labeled Goat Anti-Rabbit IgG (H+L) (1:500) secondary antibody. DAPI was used for nucleus staining. Images were acquired with a confocal laser scanning microscopy; scale bar: 10µm.