Table 2.
The effect of prednisolone and co-administration of prednisolone and a 5α-RI on glucose and lipid metabolism during a 2-step hyperinsulinemic euglycemic clamp
| Prednisolone | Prednisolone + 5α-RI | |||||
|---|---|---|---|---|---|---|
| Metabolic Variable | Before | After | Absolute Change | Before | After | Absolute Change |
| NEFAs, μmol/L | ||||||
| Basal | 490.3 ± 53.0 | 393.5 ± 67.3 | -96.8 ± 63.9 | 405.8 ± 30.3 | 465.9 ± 39.2 | 60.1 ± 45.2 |
| Low insulin | 43.1 ± 28.9 | 36.8 ± 14.3* | -6.3 ± 16.5a | 49.8 ± 8.6b | 88.5 ± 13.5b,* | 38.7 ± 10.9a |
| High insulin | 13.8 ± 4.8 | 16.3 ± 4.0 | 2.5 ± 3.3 | 22.6 ± 4.3 | 20.4 ± 3.0 | -2.2 ± 3.6 |
| Glycerol, μmol/L | ||||||
| Basal | 33.0 ± 3.1 | 29.9 ± 4.2 | -3.2 ± 4.6 | 27.2 ± 1.9 | 29.2 ± 2.6 | 2.0 ± 3.7 |
| Low insulin | 8.2 ± 1.5b | 9.9 ± 1.5b | 1.8 ± 0.3 | 7.1 ± 0.9b | 10.8 ± 1.1b | 3.7 ± 1.2 |
| High insulin | 6.9 ± 1.4 | 7.1 ± 0.9 | 0.3 ± 0.8 | 6.0 ± 0.6 | 7.4 ± 1.0 | 1.4 ± 1.0 |
| β-hydroxybutyrate, μmol/L | ||||||
| Basal | 135.2 ± 35.5 | 93.5 ± 39.0 | -41.7 ± 43.8 | 69.0 ± 14.1 | 87.8 ± 19.3 | 18.7 ± 21.1 |
| Low insulin | 15.9 ± 2.7 | 16.7 ± 1.8* | 0.8 ± 2.0 | 19.9 ± 2.1a | 25.6 ± 2.2a,* | 5.7 ± 2.1 |
| High insulin | 9.0 ± 1.2 | 10.0 ± 1.8 | 1.0 ± 2.3 | 13.6 ± 1.0 | 13.6 ± 0.9 | 0.0 ± 0.8 |
| M-value, mg/kg・minute | ||||||
| Low insulin | 3.2 ± 0.5 | 2.7 ± 0.7 | -0.5 ± 0.5 | 4.0 ± 0.5a | 2.6 ± 0.4a | -1.4 ± 0.5 |
| High insulin | 11.5 ± 1.2 | 12.0 ± 1.9 | -1.2 ± 2.0 | 10.9 ± 1.1 | 9.8 ± 0.9 | -1.1 ± 0.9 |
| M/I-value, mg/kg ・minute・pmol・L | ||||||
| Low insulin | 0.026 ± 0.005a | 0.015 ± 0.003a | -0.010 ± 0.003 | 0.026 ± 0.004a | 0.017 ± 0.002a | -0.010 ± 0.003 |
| High insulin | 0.027 ± 0.006 | 0.045 ± 0.015 | 0.015 ± 0.009 | 0.027 ± 0.004 | 0.026 ± 0.003 | -0.001 ± 0.002 |
| Ra glucose, mg/kg ・minute | ||||||
| Basal | 2.55 ± 0.34 | 2.62 ± 0.19 | 0.07 ± 0.38 | 2.67 ± 0.16a | 3.05 ± 0.18a | 0.38 ± 0.13 |
| EGP, mg/kg ・minute | ||||||
| Low insulin | 1.40 ± 0.26 | 1.29 ± 0.29 | -0.11 ± 0.36 | 1.11 ± 0.16a | 2.05 ± 0.30a | 0.93 ± 0.40 |
| Gd, mg/kg ・minute | ||||||
| Low insulin | 2.5 ± 0.6a | 1.8 ± 0.6a | -0.8 ± 0.2 | 2.9 ± 0.5 | 2.0 ± 0.3 | -0.8 ± 0.4 |
| High insulin | 11.0 ± 2.2 | 10.4 ± 2.4 | -0.6 ± 1.5 | 8.8 ± 1.1 | 8.4 ± 1.1 | -0.4 ± 1.2 |
| Ra palmitate, mg/kg ・minute | ||||||
| Basal | 1.98 ± 0.26 | 1.73 ± 0.17 | -0.26 ± 0.32 | 1.60 ± 0.09 | 1.85 ± 0.21 | 0.25 ± 0.20 |
| Low insulin | 0.66 ± 0.11 | 0.63 ± 0.09 | -0.03 ± 0.08 | 0.60 ± 0.05 | 0.96 ± 0.30 | 0.35 ± 0.26 |
| High insulin | 0.51 ± 0.06 | 0.46 ± 0.05 | -0.05 ± 0.04 | 0.49 ± 0.04 | 0.53 ± 0.08 | 0.04 ± 0.06 |
| Breath 13-CO2 AUC, mmol/h/kg | ||||||
| Low insulin | 0.042 ± 0.007 | 0.040 ± 0.004 | -0.002 ± 0.005 | 0.043 ± 0.003b | 0.036 ± 0.002b | -0.007 ± 0.002 |
| High insulin | 0.073 ± 0.003 | 0.056 ± 0.007 | -0.018 ± 0.009 | 0.070 ± 0.003c | 0.060 ± 0.002c | -0.010 ± 0.002 |
| Adipose microdialysis | ||||||
| Glycerol AUC, μmol/L・hour | ||||||
| Basal | 227.7 ± 37.1 | 242.1 ± 50.8 | 12.0 ± 23.5 | 336.4 ± 80.8 | 235.7 ± 23.2 | -77.4 ± 53.4 |
| Low insulin | 124.0 ± 38.0 | 151.7 ± 34.9 | 23.1 ± 17.5 | 199.9 ± 32.9 | 199.4 ± 39.1 | -0.4 ± 26.2 |
| High insulin | 68.0 ± 6.7 | 98.8 ± 24.9 | 25.7 ± 21.8 | 140.4 ± 34.0 | 136.8 ± 39.1 | -3.0 ± 22.6 |
| Pyruvate AUC, μmol/L・hour | ||||||
| Basal | 127.3 ± 11.4 | 132.2 ± 47.7 | 4.1 ± 35.9 | 141.8 ± 18.9 | 133.0 ± 17.2 | -6.8 ± 15.5 |
| Low insulin | 116.2 ± 17.3 | 136.0 ± 19.9 | 16.5 ± 9.6 | 144.7 ± 16.9 | 117.3 ± 21.4 | -23.2 ± 22.8 |
| High insulin | 110.6 ± 15.5 | 106.1 ± 22.6 | -3.8 ± 24.2 | 127.9 ± 22.3 | 123.2 ± 29.4 | -4.0 ± 18.0 |
| Lactate AUC, mmol/l ・hour | ||||||
| Basal | 1.7 ± 0.5 | 1.9 ± 0.7 | 0.2 ± 0.7 | 1.8 ± 0.4 | 1.5 ± 0.2 | -0.2 ± 0.3 |
| Low insulin | 3.0 ± 1.5 | 2.4 ± 0.6 | -0.5 ± 1.2 | 2.4 ± 0.5 | 1.7 ± 0.3 | -0.5 ± 0.6 |
| High insulin | 3.3 ± 1.4 | 1.9 ± 0.4 | -1.2 ± 0.9 | 2.4 ± 0.5 | 1.7 ± 0.4 | -0.5 ± 0.5 |
| Glucose AUC, mmol/l ・hour | ||||||
| Basal | 3.9 ± 0.5 | 3.7 ± 0.5 | -0.2 ± 0.6 | 4.2 ± 0.3 | 4.3 ± 0.3 | 0.1 ± 0.3 |
| Low insulin | 3.5 ± 0.7 | 3.9 ± 0.5 | 0.4 ± 0.9 | 3.3 ± 0.3 | 3.1 ± 0.4 | -0.2 ± 0.4 |
| High insulin | 3.5 ± 0.7 | 3.5 ± 0.7 | 0.0 ± 0.9 | 3.2 ± 0.4 | 2.6 ± 0.3 | -0.5 ± 0.4 |
Data shown are before, after, and absolute change following randomization to 7 days of treatment with either prednisolone (10 mg once daily) or prednisolone (10 mg once daily) and a 5aRI (finasteride [5 mg once daily] or dutasteride [0.5 mg once daily]).
Abbreviations: EGP, endogenous glucose production; Gd, glucose disposal; NEFA, nonesterified fatty acids.
a P < 0.05, before vs after treatment.
b P < 0.01, before vs after treatment.
c P < 0.001, before vs after treatment.
*P < 0.05, after vs after treatment.