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. 2020 Sep 8;14(9):e0008365. doi: 10.1371/journal.pntd.0008365

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© 2020 Norris et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (a) Chemical synthesis scheme of Rho functionalization by melt condensation. (b) ¹H NMR spectra of Rho-functionalized polymers and precursors. (c) NP-HPLC chromatograms of Rho-functionalized polymers for quantifying attached vs unattached Rho. Attached Rho elutes at 3.7 min and unattached Rho elutes at 5 min. Rho-functionalized polymers appear to contain significant amounts of unattached Rho.