Table 2. List of antibodies used in western blot.

Protein target	UniProt ID and Gene Name	Primary antibody	Secondary antibody
Ubiquitin	Not applying—the antibody targets ubiquitin and polyubiquitin chains	Mouse mAb 1:500; sc-166553	Peroxidase-AffiniPure Goat pAb anti-mouse 1:10000; Jackson Immunoresearch lab. 115-035-062
Muscle Ring Finger 1	Q969Q1 TRIM63	Goat pAb 1:200; ab4125	HRP-linked Donkey pAb anti-goat 1:10000; sc-2020
Muscle Ring Finger 3	Q9BYV2 TRIM54	Goat pAb 1:500; ab4351	HRP-linked Donkey pAb anti-goat 1:10000; sc-2020
Atrogin-1	Q969P5 FBXO32	Mouse mAb 1:500; sc-166806	DyLight 800-conjugated Goat pAb anti-mouse; Bio-Rad STAR117D800GA
Murine Double Minute 2	Q00987 MDM2	Mouse mAb 1:500; sc-965	Peroxidase-AffiniPure Goat pAb anti-mouse 1:10000; Jackson Immunoresearch lab. 115-035-062

Abbreviations: HRP: horseradish peroxidase; mAb: monoclonal antibody; pAb: polyclonal antibody