Table 1.
Box–Behnken design applied from RSM for hemp seed oil extraction
| Run | Independent variables | Responses | ||||||
|---|---|---|---|---|---|---|---|---|
| Solvent ratio (v/v%) | Temperature (°C) | Time (min) | Oil yield (%) | Total phenol (mg GAE/g) | DPPH inhibition (%) | Peroxide (meq/kg) | Color index | |
| 1 | 0 | −1 | −1 | 23.8 | 2.84 | 65 | 1.8 | 30.4 |
| 2 | −1 | −1 | 0 | 26 | 3.49 | 76.8 | 3 | 43.5 |
| 3 | 0 | 1 | −1 | 22.8 | 2.5 | 60.2 | 4.5 | 34.3 |
| 4 | 0 | 0 | 0 | 31.7 | 3.24 | 76.1 | 6 | 35.3 |
| 5 | 0 | 0 | 0 | 31.5 | 3.39 | 75.8 | 6 | 35 |
| 6 | 1 | 1 | 0 | 26.2 | 2 | 53.5 | 6.9 | 8.3 |
| 7 | −1 | 0 | −1 | 23.3 | 3.04 | 71.5 | 2.7 | 41.2 |
| 8 | 0 | 0 | 0 | 31.6 | 3.4 | 76.5 | 5.4 | 32.3 |
| 9 | 1 | 0 | 1 | 30 | 2.2 | 54.5 | 9 | −3.1 |
| 10 | 1 | 0 | −1 | 26.4 | 2.05 | 54.9 | 2.4 | −0.8 |
| 11 | 0 | −1 | 1 | 28 | 2.74 | 67.2 | 7.5 | 31.9 |
| 12 | 0 | 1 | 1 | 25 | 2.6 | 59 | 9.6 | 36.8 |
| 13 | 1 | −1 | 0 | 27.2 | 2.15 | 58.7 | 3.9 | 4 |
| 14 | −1 | 1 | 0 | 23.2 | 2.95 | 69.7 | 6.3 | 49.4 |
| 15 | −1 | 0 | 1 | 27.2 | 3.3 | 75.2 | 7.6 | 46.6 |
| Independent variables | Unit | Symbol | Levels | ||
|---|---|---|---|---|---|
| −1 | 0 | +1 | |||
| Solvent ratio (hexane: isopropanol) | v/v % | A | 0:100 | 50:50 | 100:0 |
| Temperature | °C | B | 30 | 45 | 60 |
| Time | min | C | 30 | 60 | 90 |