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. Author manuscript; available in PMC: 2020 Sep 18.
Published in final edited form as: Mol Psychiatry. 2018 Sep 13;25(4):750–760. doi: 10.1038/s41380-018-0234-y

Fig 2A-B.

Fig 2A-B.

Fig 2A. mGluR5 phosphorylation is altered in schizophrenia. DLPFC slices from 17 matched pairs were incubated with 0, 0.1 or 1 µM of CHPG for 15 min. Protein extracts were immunoprecipitated with anti-mGluR5 in a denaturing condition, which were then probed for phosphor-serine (pS), -threonine (pT) and -tyrosine (pY). Fig 2B. Phosphorylation of mGluR5 can reduce coupling of mGluR5 to its diverse binders. Cortical slices were treated with alkaline phosphatase to eliminate phosphorylation, which were then incubated with 0, 0.1 or 1 µM of CHPG for 15 min. Tissue lysates were IPed for mGluR5 complexes and probed for mGluR5 binders including Gαq/11, Homer, PI3K, RGS4 and GluN2A by immunoblotting. N = 9