Figure 5.
Bioconditioned scaffolds upregulate expression of key osteogenic markers. (A) Quantitative RT-PCR analysis showed an upregulation of Coll VI and Coll XII in response to GW9662 treatment, but not to bioconditioned scaffolds after 8 days of culture. (B) An increase at the transcription level of BMP2, osteocalcin, and osteopontin was observed after 21 days of culture on bioconditioned scaffolds. Fold changes were normalized to the expression levels of uninduced hMSCs after 8 or 21 days of 2D culture. A one-way ANOVA, followed by a Tukey’s multiple comparison test was performed on ΔΔCT values (* p<0.05).