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PCR Mix to amplify and sgRNA 2
| Component | Amount |
|---|---|
| Nuclease-free dH2O | Up to 50μl |
| 5x Phusion HF Buffer | 10μl |
| 10mM dNTPs | 1μl |
| DMSO (optional) | 1.5μl |
| 10uM Forward Primer | 2.5μl |
| 10uM Reverse Primer | 2.5μl |
| Template DNA (in H2O) | 100ng |
| Phusion DNA Polymerase | 0.5μl |
