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. Author manuscript; available in PMC: 2021 Apr 7.
Published in final edited form as: Biochemistry. 2020 Mar 18;59(13):1328–1337. doi: 10.1021/acs.biochem.0c00097

Figure 4.

Figure 4.

Portion of the 1H NMR spectrum of GDP-d-glycero-α-d-manno-heptose (1) and GDP-d-glycero-4-keto-α-d-lyxo-heptose (2). (a) Control 1H NMR spectrum of GDP-d-glycero-α-d-manno-heptose. (b) 1H NMR spectrum of GDP-d-glycero-α-d-manno-heptose (2.0 mM) after the addition of Cj1427 (20 μM) for 1 h in the presence of 20 mM α-ketoglutarate in 50 mM potassium phosphate buffer, pH 8.0. Peak assignments were made from previously publications (22, 24, 25). Integrations are shown in shaded area for each resonance.