Table 1.
Steady-state kinetic parameters for Cj1427a
| Enzyme | Substrate | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
| Cj1427 | GDP-d-glycero-α-d-manno-heptoseb | 0.64 ± 0.02 | 63 ± 5 | (10.2 ± 0.8) × 103 |
| Cj1427 | α-ketoglutaratec | 0.55 ± 0.01 | 130 ± 8 | (4.3 ± 0.3) × 103 |
a
Errors were calculated from the standard deviation of the fitting results. Reactions were monitored by UV coupled assay at 340 nm in 50 mM HEPES/KCl, pH 7.4 at 30 °C in the presence of 300 μM NADPH and 10 μM Cj1430 and Cj1428.
b
The concentration of GDP-d-glycero-α-d-manno-heptose was varied with a fixed concentration of α-ketoglutarate (1.0 mM).
c
Concentration of α-ketoglutarate was varied with a fixed concentration GDP-d-glycero-d-manno-heptose (0.5 mM).