Table 2.

Individual Rate Constants and Errors^a

	Lower limitb	Upper Limitb	% rangec	Best fit
f/f0d	0.23	0.349	21%	0.29
Kde (μM)	140	200	18%	165
k3 (s−1)	97.2	140	18%	116
k4 (s−1)	11.5	14.2	11%	12.8

^aThe data are fit globally to the mechanism shown in Scheme 4.

^bThe upper and lower limits reflect a threshold of a 10% deviation from the minimal SSE in the confidence contours. FitSpace error confidence contours are shown in Figure 8 (19).

^cThe percentage range was calculated by dividing the mean of the range by the best fit value as (upper − lower)/(2 × best fit). This reflects the allowable variation of each best fit value as a percentage.

^dThe fluorescence scaling factor is shown as the fractional change in enzyme fluorescence (see the text).

^eThe value for k₁ (α-ketoglutarate binding) was assumed to be fast and held fixed at a lower limit of 5.5 × 10⁶ M⁻¹ s⁻¹, so fitting to derive k₂ defined only K_d.