Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Gria1 | GenBank | #14799 | |
| Strain, strain background (Mus musculus, strain C57BL6) | HA-ΔCTD GluA1 | This paper | N/A | |
| Strain, strain background (Mus musculus, strain FVB) | Rosa26-Cas9 KI | The Jackson Laboratory | #026558; RRID:IMSR_JAX:026558 | |
| Strain, strain background (Mus musculus, strain C57BL6) | Gria1-3f/f | Lu et al., 2009 | N/A | |
| Strain, strain background (Rattus norvegicus, strain CD Sprague Dawley IGS) | WT | Charles River | #001 | |
| Cell line (Homo sapiens) | 293T | ATCC | #CRL-3216; RRID:CVCL_0063 | |
| Recombinant DNA reagent (plasmid) | pCAGGS-GluA1/A2 CTD-IRES-GFP | This paper | N/A | Expression of chimeric GluA1/A2 CTD and GFP (under IRES). |
| Recombinant DNA reagent (plasmid) | pCAGGS-GluA1/A2 CTD-IRES-mCherry* | This paper | N/A | Gria1 CRISPR-resistant expression of chimeric GluA1/A2 CTD and mCherry (under IRES) |
| Recombinant DNA reagent (plasmid) | pCAGGS- IRES-mCherry | Incontro et al., 2014 | N/A | Expression of mCherry (under IRES) |
| Recombinant DNA reagent (plasmid) | pFUGW-Cre:GFP | Díaz-Alonso et al., 2017 | N/A | Expression of Cre:GFP fusion protein |
| Recombinant DNA reagent (plasmid) | px458- Gria1-CRISPR | This paper | N/A | Expression of Gria1 gRNA, Cas9 and GFP. Derived from px458 vector (Addgene #48138 RRID:Addgene_48138) |
| Recombinant DNA reagent (plasmid) | px458- Grin1-CRISPR | Incontro et al., 2014 | N/A | Expression of Grin1 gRNA, Cas9 and GFP. Derived from px458 vector (Addgene #48138); RRID:Addgene_48138 |
| Recombinant DNA reagent | ssDNA encoding HA tag and stop codons flanked by 60 bp long homology arms for HDR | This paper | N/A | Obtained from IDT. Injected in fertilized zigotes for HA-ΔCTD GluA1 KI mouse generation (see Materials and methods for sequence) |
| Other (Recombinant RNA reagent) | Gria1 1 gRNA | This paper | N/A | Obtained from IDT. Injected in fertilized zigotes for HA-ΔCTD GluA1 KI mouse generation (see Materials and methods for sequence) |
| Other (Recombinant RNA reagent) | Gria1 2 gRNA | This paper | N/A | Obtained from IDT. Injected in fertilized zigotes for HA-ΔCTD GluA1 KI mouse generation (see Materials and methods for sequence) |
| Antibody | Rabbit polyclonal anti-GluA1 C-tail | Synaptic Systems | #182–003; RRID:AB_2113441 | IF (1:500) WB (1:1000) |
| Antibody | Rabbit polyclonal anti-GluA2 C-tail | Synaptic Systems | #182–103; RRID:AB_2113732 | WB (1:1000) |
| Antibody | Mouse monoclonal anti-GluA1 ATD | Millipore | #MAB 2263; RRID:AB_11212678 | WB (1:1000) |
| Antibody | Mouse, monoclonal anti-NR1 | Millipore | #05–432; RRID:AB_390129 | WB (1:1000) |
| Antibody | Rabbit polyclonal anti-HA | Thermo Fisher Scientific | #71–5500; RRID:AB_2533988 | WB (1:1000) |
| Antibody | Rabbit polyclonal anti-alpha tubulin | Cell Signaling | #2144; RRID:AB_2210548 | WB (1:1000) |
| Antibody | HRP conjugated anti-mouse secondary antibody | GE Healthcare | #NA931; RRID:AB_772210 | WB (1:5000) |
| Antibody | HRP conjugated anti-rabbit secondary antibody | GE Healthcare | #NA934; RRID:AB_772206 | WB (1:5000) |
| Antibody | Alexa-488 conjugated anti-rabbit secondary antibody | Thermo Fisher Scientific | #A11034; RRID:AB_2576217 | IF (1:500) |
| Chemical compound, drug | D(-)−2-amino-5-phosphonovaleric acid (AP5) | Hello Bio | #HB0225 | 0.1 mM |
| Chemical compound, drug | Picrotoxin | TCI | #C0375 | 0.1 mM |
| Chemical compound, drug | Bicuculline | Sigma-Aldrich | #14340 | 0.02 mM |
| Chemical compound, drug | 2-Chloroadenosine | Sigma-Aldrich | #C5134 | 2 mM |
| Commercial assay, kit | Helios Gene Gun Kit | Bio-Rad | #1652411 | Used for biolistic transfection of hioppocampal slice cultures |
| Commercial assay, kit | In fusion HD cloning kit | Takara Bio | #639647 | Used for clonning of GluA1/A2 CTD in pCAGGS vectors |
| Commercial assay, kit | NheI | New England Biolabs | #R0131 | Restriction enzyme. Used for clonning of GluA1/A2 CTD in pCAGGS vectors |
| Commercial assay, kit | XhoI | New England Biolabs | #R0146 | Restriction enzyme. Used for clonning of GluA1/A2 CTD in pCAGGS vectors |
| Commercial assay, kit | BbsI | New England Biolabs | #R3539 | Restriction enzyme. Used for clonning of gRNA in px458 vectors |
| Commercial assay, kit | T4 DNA ligase | New England Biolabs | #M0202L | Ligase. Used for clonning of gRNA in px458 vectors |
| Commercial assay, kit | MycoAlert PLUS Mycoplasma Detection Kit | Lonza | #LT07-701 | Mycoplasma contamination assay |
| Commercial assay, kit | Lipofectamine 2000 | Thermo Fisher Scientific | #11668027 | Transfection reagent for 293 T cells |
| Software, algorithm | Prism | Graph Pad | https://www.graphpad.com/scientific-software/ prism/; RRID:SCR_002798 | |
| Software, algorithm | Igor Pro | Wavemetrics | https://www.wavemetrics.com/products/igorpro; RRID:SCR_000325 | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/; RRID:SCR_003070 |