Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Chlamydomonas reinhardtii) | WT | Chlamydomonas Resource Center | cMJ030 | Wild-type |
| Strain, strain background (Paramecium tetraurelia) | 7S | Beisson et al., 2010 | doi:10.1101/pdb.prot5364 | |
| Cell line (Homo sapiens) | U2OS | Habedanck et al., 2005 | PMID:16244668 | |
| Cell line (Homo sapiens) | RPE-1 p53- | Wang et al., 2015 | PMID:26609813 | |
| Cell line (Homo sapiens) | U2OS:GFP-WDR90RR | This paper | p. 19 of the manuscript (Material and methods) | Episomal, puromycine selected, doxycycline-inducible |
| Transfected construct (Homo sapiens) | GFP-WDR90RR | This paper | pEBTet-GFP-WDR90RR(FL) p. 20 of the manuscript (Material and methods) |
WDR90RR DNA template from Hamel et al., 2017 |
| Transfected construct (Homo sapiens) | GFP-WDR90(1-225)RR | This paper | pEBTet-GFP-WDR90RR(1-225) p. 20 of the manuscript (Material and methods) |
WDR90RR DNA template from Hamel et al., 2017 |
| Transfected construct (Homo sapiens) | GFP-WDR90 | This paper | Genebank sequence NP_660337, pEGFP-WDR90 | RT-PCR from human RPE-1 cells, cloned into modified pEGFP-C1 vector using AscI andPacI restriction sites |
| Transfected construct (Chlamydomonas reinhardtii) | POC16 (1-295) | This paper | pXLG-POC16(1-295), described p.20 of the manuscript in the Material andmethods section. | POC16 sequence synthetized by GeneArt using the E. coli codon usage (described in Hamel et al., 2017) cloned into pXLG vector using NotI and BamHI restriction sites |
| Biological sample (Chlamydomonas reinhardtii) | Isolated basal bodies | Klena et al., 2018 | PMID:30295659 | |
| Biological sample (Sus scrofa) | Tubulin | Cytoskeleton | Cat. #: T240 | Isolated from brain, used for electron microscopy |
| Biological sample (Bovine taurus) | Tubulin | Centro de Investigastiones Biologicas, Madrid, Spain | Isolated from brain, used for pelleting assay | |
| Antibody | Tubulin AA345 (mouse monoclonal) | Le Guennec et al., 2020 | PMID:32110738 | U-ExM Isolated Basal Bodies (1:500) U-ExM in cells (1:250) |
| Antibody | Alpha-Tubulin AA344 (mouse monoclonal) | Le Guennec et al., 2020 | PMID:32110738 | U-ExM in cells (1:250) |
| Antibody | POC16 (rabbit polyclonal) | Hamel et al., 2017 | PMID:28781053 | U-ExM (1:100) |
| Antibody | POB15 (rabbit polyclonal) | Hamel et al., 2017 | PMID:28781053 | U-ExM (1:100) |
| Antibody | WDR90 (rabbit polyclonal) | NovusBio | Cat. #: NBP2-31888 | U-ExM (1:100) IF (1:250) |
| Antibody | POC1B (rabbit polyclonal) | ThermoFisher | Cat. #: PA5-24495 | U-ExM (1:250) |
| Antibody | POC5 (rabbit polyclonal) | Bethyl | Cat. #: A303-341A | U-ExM (1:250) IF (1:500) |
| Antibody | FAM161A (rabbit polyclonal) | Le Guennec et al., 2020 | PMID:32110738 | U-ExM (1:250) |
| Antibody | Centrin (mouse monoclonal, 20H5) | Merck Millipore | Cat. #: 04–1624 | U-ExM (1:250) IF (1:500) |
| Antibody | DM1A Tubulin (mouse monoclonal) | Abcam | Cat. #: ab7291 | IF (1:1000) |
| Antibody | HsSAS-6 (mouse monoclonal) | Santa Cruz Biotechnology | Cat. #: sc-81431 | IF (1:100) |
| Antibody | PCM1 (rabbit polyclonal) |
Santa Cruz Biotechnology | Cat. #: sc-67204 | IF (1:500) |
| Antibody | CP110 (rabbit polyclonal) |
Proteintech | Cat. #: 12780–1 | IF (1:500) |
| Antibody | GFP (mouse monoclonal) | Abcam | Cat. #: ab1218 | IF (1:500) |
| Antibody | mCherry (rabbit polyclonal) | Abcam | Cat. #: ab167453 | IF (1:500) |
| Antibody | ptPOC16 (rabbit polyclonal) |
This study | described p.27 of the manuscript in the Supplemental Methods section. | IF (1:50) |
| Antibody | Tubulin 1D5 (mouse mono clonal) |
Beisson et al., 2010 | IF (1:10) | |
| Antibody | Alexa 488 anti-rabbit IgG (goat) | ThermoFisher | Cat. #: A11008 | U-ExM (1:400) IF (1:1000) |
| Antibody | Alexa 568 anti-mouse IgG (goat) | ThermoFisher | Cat. #: A11004 | U-ExM (1:400) IF (1:1000) |
| Recombinant DNA reagent | pEBTet-EGFP-GW | Gift from the Gönczy lab | Na. | |
| Recombinant DNA reagent | pENTR-Age-AGT | Gift from the Gönczy lab | Na. | |
| Recombinant DNA reagent | pEGFP-C1 | Clontech | ||
| Sequence-based reagent | siRNA Control | ThermoFisher | AM4642 | Silencer select |
| Sequence-based reagent | siRNA targetingwdr90 gene | ThermoFisher | S47097 | Silencer select |
| Sequence-based reagent | siRNA targetingpcm1 gene | ThermoFisher | ADCSU9L | Silencer select |
| Peptide, recombinant protein | POC16(1-295) | This paper | Uniprot A8JAN3 | Purified from bacteria |
| Peptide, recombinant protein | WDR90(1-225) | This paper | Uniprot Q96KV7 | Purified from bacteria |
| Peptide, recombinant protein | drPOC16(1-243) | This paper | Uniprot F1RA29 | Purified from bacteria |
| Peptide, recombinant protein | btPOC16(1-224) | This paper | Uniref UPI000572B175 | Purified from bacteria |
| Peptide, recombinant protein | ptPOC16(2-210) | This paper | Uniprot A0DK60 | Purified from bacteria |
| Peptide, recombinant protein | xtPOC16(1-245) | This paper | Uniref UPI0008473371 | Purified from bacteria |
| Peptide, recombinant protein | rnPOC16(54-282) | This paper | Uniref UPI0008473371 | Purified from bacteria |
| Commercial assay or kit | Lipofectamine 3000 Transfection kit | LifeTechnology | Cat. #: L3000015 | |
| Commercial assay or kit | Lipofectamine RNAi max kit | LifeTechnology | Cat. #: 13778150 | |
| Commercial assay or kit | Click-EdU-Alexa647 FACS kit | Carl Roth | Cat. #: 7783.1 | |
| Commercial assay or kit | DAPCO Mounting medium | Abcam | Cat. #: ab188804 | |
| Commercial assay or kit | Affi-Gel 10 | Bio-Rad | Cat. #:153–6099 | |
| Chemical compound, drug | Formaldehyde 36.5–38% | Sigma | Cat. #: F8775 | |
| Chemical compound, drug | Acrylamide 40% | Sigma | Cat. #: A4058 | |
| Chemical compound, drug | N,N’-methylbisacrylamide 2% | Sigma | Cat. #: M1533 | |
| Chemical compound, drug | Sodium acrylate 97–99% | Sigma | Cat. #: 408220 | |
| Chemical compound, drug | Ammonium persulfate | ThermoFisher | Cat. #: 17874 | |
| Chemical compound, drug | Tetramethylethyldiamine | ThermoFisher | Cat. #: 17919 | |
| Chemical compound, drug | Poly-D-Lysine 1 mg/mL | Gibco | Cat. #: A3890401 | |
| Chemical compound, drug | Taxol/Paclitaxel | Sigma-Aldrich | Cat. #: T7191 | |
| Chemical compound, drug | Coomassie staining | Biotium | Cat. #: 21003 | |
| Chemical compound, drug | Propidium Iodide | Sigma | Cat. #: 81845 | |
| Chemical compound, drug | Rnase | Roche | Cat. #: 11119915001 | |
| Software, algorithm | ImageJ/FiJi | Schindelin et al., 2012 | doi:10.1038/nmeth.2019 | |
| Software, algorithm | CentrioleJ pluggin | Guichard et al., 2013 | DOI:10.1016/j.cub.2013.06.061 | |
| Software, algorithm | UnwarpJ pluggin | Sorzano et al., 2005 | DOI:10.1109/TBME.2005.844030 | |
| Software, algorithm | GraphPadPrism7 | GraphPad Software | 7.0 | |
| Software, algorithm | Phyre2 | Kelley and Sternberg, 2009 | DOI:10.1038/nprot.2015.053 | |
| Software, algorithm | UCSF Chimera | Pettersen et al., 2004 | DOI:10.1002/jcc.20084 | |
| Other | Zeiss LSM700 microscope | Zeiss | ||
| Other | Leica TCS SP8 microscope | Leica | Expansion microscopy | |
| Other | Leica Thunder inverted microscope | Leica | ||
| Other | Tecnai G2 Sphera microscope | Thermofisher | Negative stain and cryo-EM |