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. 2020 Feb 17;27(9):427–434. doi: 10.1038/s41434-020-0125-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Blood samples were collected from microminipigs (n = 48) from a single breeding farm. Serum was prepared from blood samples and stored at −20 °C until measurement. a The anti-AAV8–neutralizing antibody (NAb) titer is expressed as the final dilution of the test serum in the assay. b The level of specific anti-AAV8 immunoglobulins present in the serum was evaluated by ELISA. The relationship between the amount of anti-AAV8 antibody and NAb titer was analyzed. Dilution titer of the serum was 1:2000. Data are shown as mean ± SD. One-way ANOVA test was used to determine statistical significance.