Fig. 8.

Determination of systemic virus loads in several organs of C57BL/6 Stat2^−/− mice infected with ZIKV PRVABC59 in the presence of α-DSV4, α-Dengvaxia and α-TV DENV immune sera. A subset of mice (n=3) from the different immune sera passive transfer groups in the experiment described in Fig. 7, were euthanised on day 6 following ZIKV infection. Equal amounts of total RNA, extracted from the indicated tissues of these mice, were reverse-transcribed to cDNA and subjected to qPCR. Viral RNA genomes were quantified with reference to an in vitro-transcribed RNA standard. Each data point denotes tissue RNA level in a single mouse. The horizontal lines represent the mean tissue virus load. The double asterisks indicate statistical significance (two-way ANOVA with Bonferroni correction) of the data for group above which they are placed compared to that of the ‘Mock’ group. All other groups did not differ significantly in comparison to the mock group.