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. 2020 Aug 22;37(5):577–588. doi: 10.1007/s10719-020-09941-z

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Fig. 3 — Secretion of different B4GalT4 versions. HEK293T cells stably overexpressing variants of B4GalT4 were cultured in Opti-MEM without FBS for 24 h. Conditioned media were concentrated by centrifugation in Amicon Pro 10 kDa cut-off membranes and analyzed by Western blotting with anti-B4GalT4 and anti-Mgat5 antibodies for protein assessment and loading control, respectively