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. 2020 Aug 22;37(5):577–588. doi: 10.1007/s10719-020-09941-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Assessment of glycan impact on interaction between B4GalT4 and SLC35A2. Results of the NanoBiT assay performed for the selected combinations and the corresponding negative control. B4GalT4-deficient HEK293T cell cultures were transfected with combinations of plasmids bearing either small or large subunit of the NanoLuc luciferase fused to studied proteins or a negative control (HaloTag fused with the small NanoLuc subunit). A2 – SLC35A2 tagged with the large NanoLuc subunit at the N-terminus; B4GalT4, M2, M3 and M23 – respective B4GalT4 variants tagged with the small NanoLuc subunit at the N-terminus, NEG -– HaloTag fused with the small NanoLuc subunit, RLU – relative luminescence unit. p < 0.0001 ****(b) Fold changes calculated by dividing the average luminescence obtained for the tested combinations (Sample RLU) by the average luminescence obtained for the corresponding negative control (Control RLU). The threshold value was set at 10, according to the manufacturer’s suggestions