Extended Data Fig. 9: Ubiquitination of ZIKV-E promotes virus-endosome membrane fusion in A549 cells.

a-d, ZIKV E-WT, E-K38R, and E-K281R were labeled with DiOC18. After filtration, viruses were incubated at 4°C with A549 cells at MOI 2 and after 30min were washed and collected for controls as adsorbed viruses. Additional samples were then incubated at 37°C for 1h, and NH₄Cl was used as control to block acidification of the endosome. Cells were washed, fixed, and visualized in a confocal microscope in a. Quantification by FACS is shown (mean fluorescence intensity, MFI) in b-c. d, Representative histograms showing green florescence (FL1: DiOC18) during ZIKV-endosome fusion. N=3, technical replicates, mean, unpaired two-sided t-test, *P < 0.05, ***P < 0.05. Representatives of 2 independent experiments.