Skip to main content
. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Metab Eng. 2020 Jul 22;61:352–359. doi: 10.1016/j.ymben.2020.07.004

Figure 2. Acyl-CoA Synthetase with activity on octanoic acid.

Figure 2.

(A) E. coli MHS04 (MG1655 ΔfadR ΔfadD) cells carrying plasmids with various acyl-CoA synthases are tested for their ability to convert octanoic acid to acetyl-CoA via deregulated β-oxidation. (B) MHS04 carrying ACS plasmids were grown in LB media with 250 mg/L octanoic acid (see materials and methods), and the octanoic acid consumption was tracked over time. MtFadD6 was the best performer with most of the octanoic acid being consumed after 5 hours.