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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: FASEB J. 2020 Jun 2;34(7):9694–9712. doi: 10.1096/fj.202000667R

Figure 2). Specific targeting of gap junction gene transcription during adenovirus type 5 infection.

Figure 2)

HaCaT cells were infected with Ad5 at a MOI of 10 iu/cell and RNA was harvested at 24 hpi. A) RT-qPCR analysis of CDKN1A (p21) and SFN (14-3-3σ) to confirm active viral infection and known altered gene transcription. B) RT-qPCR analysis of gap junction family genes GJA1 (Cx43), GJA5 (Cx40), and GJC1 (Cx45). C) RT-qPCR analysis of junctional protein genes PKP2 (Plakophilin-2) and TJP1 (ZO-1). Statistical analysis performed by one-way ANOVA with Dunnett’s multiple comparisons test. (n=3). *p≤0.05 **p≤0.01 ***p≤0.001 ****p<0.0001. Data are represented as mean ±SEM. See also Figure S1.