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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: FASEB J. 2020 Jun 2;34(7):9694–9712. doi: 10.1096/fj.202000667R

Figure 5). Cx43 protein occurs in reduced levels at the endoplasmic reticulum and is primarily junctional during adenovirus infection.

Figure 5)

HaCaT cells were infected with AdlacZ or Ad5 at a MOI of 10 iu/cell prior to fixation or protein harvesting at 24 hpi. A) AdlacZ- or Ad5-infected HaCaT cells were immunolabeled against PDI (red) to localize ER and against Cx43 (green). Cells were stained using DAPI to identify nuclei (blue). Colocalized Cx43 / PDI signal was determined with ImageJ (white). Original magnification: X100. Scale bar: 20 μm. B) Manders’ Coefficients calculations determined using fraction Cx43 with PDI. Data points represent averages of 8 images from 3 separate experiments. C) Pearson’s Coefficients calculations determined for Cx43 and PDI correlation. Data points represent averages of 8 images from 3 separate experiments. D) AdlacZ- or Ad5-infected HaCaT cells were lysed in 1% Triton X-100 solubility assay buffer prior to fractionation and western blotting. Membrane probed against Cx43 (top panel) and α-tubulin (bottom panel) for loading control. E) Quantification of D. Statistical analysis was performed with Student’s t-test. (n=3). *p≤0.05, **p≤0.01. Data are represented as mean ±SEM.