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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: FASEB J. 2020 Jun 2;34(7):9694–9712. doi: 10.1096/fj.202000667R

Figure 6). Adenovirus inhibits gap junction intercellular communication prior to reduction of Cx43 total protein levels during infection.

Figure 6)

HaCaT cells were plated to confluence on glass bottomed dishes and infected with Ad5 or replication-incompetent AdlacZ at a MOI of 10 iu/cell. The Lucifer yellow (LY) scrape-load dye-transfer assay was performed at 12 hpi with 10,000 MW Dextran-AlexaFluor647 (Dex-647) as a gap junction non-permeable control. A) Representative confocal microscopy images of LY (green) and Dex-647 (red) transfer with nuclei identified with DAPI (blue). Middle greyscale panels show LY and Dex-647 alone and right panels illustrate quantification of each dye spread. Original magnification: X20. Scale bar: 50μm. B) Quantification of dye spread in A. Statistical analysis was performed with the unpaired Student’s t-test. (n=4). ****p<0.0001. Data are represented as mean ±SEM. See also Figure S4.