Extended Data Table 1.
Human cell lines used in this study.
| Parental cell lines | ||||
|---|---|---|---|---|
| OD cell line code | Name | Source | Clonal or Polyclonal | Catalog # |
| RCL001 | hTERT RPE-1 | ATCC | n/a | CRL-4000 |
| RCL008 | CHP212 | ATCC | n/a | CRL-2273 |
| RCL010 | IMR32 | ATCC | n/a | CCL-127 |
| RCL019 | CHP134 | Sigma | n/a | 06122002 |
| RCL020 | SK-N-F1 | ATCC | n/a | CRL-2142 |
| RCL021 | HepG2 | ATCC | n/a | HB-8065 |
| RCL022 | MCF7 | ATCC | n/a | HTB-22 |
| RCL023 | BT-474 | ATCC | n/a | HTB-20 |
| RCL024 | BT-549 | ATCC | n/a | HTB-122 |
| RCL025 | MDA-MB-231 | ATCC | n/a | HTB-26 |
| RCL026 | Freestyle 293-F | Thermo Fisher Scientific | n/a | R79007 |
| RCL027 | KPNYN | Gift from Peter Zage (UCSD) | n/a |
| Engineered cell lines | ||||
|---|---|---|---|---|
| OD cell line code | Parental line | Modification(s) | Clonal or Polyclonal | Reference |
| ODCL0002 | hTERT RPE-1 | USP28Δ | Clonal | Meitinger et al. 2016 |
| ODCL0003 | hTERT RPE-1 | CEP192-mNeonGreen | Clonal | This study |
| ODCL0035 | hTERT RPE-1 | EF-1αpro-H2B-mRFP | Polyclonal | Meitinger et al. 2016 |
| ODCL0036 | CHP212 | EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0049 | CHP134 | EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0060 | SK-N-F1 | EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0061 | hTERT RPE-1 | TRIM37Δ | Clonal | Meitinger et al. 2016 |
| ODCL0062 | ODCL0061 | TRIM37Δ UbCpro-TRIM37–3FLAG | Polyclonal | This study |
| ODCL0063 | ODCL0061 | TRIM37Δ UbCpro-TRIM37-W373A-3FLAG | Polyclonal | This study |
| ODCL0064 | ODCL0061 | TRIM37Δ UbCpro-TRIM37-C18R-3FLAG | Polyclonal | This study |
| ODCL0065 | ODCL0061 | TRIM37Δ UbCpro-TRIM37-C18R-W373A-3FLAG | Polyclonal | This study |
| ODCL0068 | ODCL0061 | TRIM37Δ PGKpro-TRIM37-C18R-mNeonGreen | Polyclonal | This study |
| ODCL0070 | ODCL0068 | TRIM37Δ PGKpro-TRIM37-C18R-mNeonGreen | Polyclonal | This study |
| EF-1αpro-mRuby2-MAP4-MBD | ||||
| ODCL0071 | ODCL0003 | CEP192-mNeonGreen; USP28Δ | Clonal | This study |
| ODCL0072 | ODCL0071 | CEP192-mNeonGreen; USP28Δ; EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0073 | ODCL0003 | CEP192-mNeonGreen; TRIM37Δ | Clonal | This study |
| ODCL0074 | ODCL0073 | CEP192-mNeonGreen; TRIM37Δ; EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0075 | ODCL0071 | CEP192-mNeonGreen; USP28Δ; EF-1αpro-mRuby2-MAP4-MBD | Polyclonal | This study |
| ODCL0076 | ODCL0073 | CEP192-mNeonGreen; TRIM37Δ; EF-1αpro-mRuby2-MAP4-MBD | Polyclonal | This study |
| ODCL0077 | ODCL0071 | CEP192-mNeonGreen; USP28Δ; TRE3Gpro-Cas9 | Clonal | This study |
| ODCL0078 | ODCL0073 | CEP192-mNeonGreen; TRIM37Δ; TRE3Gpro-Cas9 | Clonal | This study |
| ODCL0079 | ODCL0077 | CEP192-mNeonGreen; USP28Δ; TRE3Gpro-Cas9; U6pro-gRNA-PLK4 | Polyclonal | This study |
| ODCL0080 | ODCL0078 | CEP192-mNeonGreen; TRIM37Δ; TRE3Gpro-Cas9; U6pro-PLK4-gRNA | Polyclonal | This study |
| ODCL0081 | RCL001 | TRE3GSpro-TRIM37 | Clone 3; ED Fig. 1b–f | This study |
| ODCL0082 | RCL001 | TRE3GSpro-TRIM37 | Clone 4; Fig. 3g, ED Fig. 1b–f | This study |
| ODCL0083 | RCL001 | TRE3GSpro-TRIM37 | Clone 5; Fig. 1a–b, 3g, ED Fig. 1b–f | This study |
| ODCL0084 | ODCL0061 | TRIM37Δ; TRE3GSpro-TRIM37 | Clone 1; ED Fig. 1b–f | This study |
| ODCL0085 | ODCL0061 | TRIM37Δ; TRE3GSpro-TRIM37 | Clone 2; ED Fig. 1b–f | This study |
| ODCL0086 | ODCL0003 | CEP192-mNeonGreen; TRE3GSpro-TRIM37 | Clonal | This study |
| ODCL0087 | ODCL0086 | CEP192-mNeonGreen; TRE3GSpro-TRIM37; EF-1αpro-H2B-mRFP | Polyclonal | This study |
| ODCL0088 | CHP134 | TRIM37+/−/−/− (17bp del; 7bp del; 1bp ins) | Clone 1; Fig. 1f, 3f, ED Fig. 2h | This study |
| ODCL0089 | CHP134 | TRIM37+/+/+/− (374bp del) | Clone 6; Fig. 1f, ED Fig. 2h | This study |
| ODCL0090 | CHP134 | TRIM37+/−/−/− (15bp del; 1bp ins; 7bp ins) | Clone 4; Fig. 1f, ED Fig. 2h | This study |
| ODCL0091 | CHP134 | TRIM37+/+/+/− (2bp del) | Clone 5; Fig. 1f, ED Fig. 2f | This study |
| ODCL0092 | CHP134 | TRIM37+/−/−/− (1bp del; 1bp del; 17bp ins) | Clone 2; Fig. 1f, ED Fig. 2h | This study |
| ODCL0093 | CHP134 | TRIM37+/+/+/− (3 copies wildtype; mutation unclear) | Clone 3; Fig. 1f, ED Fig. 2h | This study |
| ODCL0099 | CHP134 | hEF1a/TurboGFP inducible PLK4-shRNA | Polyclonal | This study |
| ODCL0108 | ODCL0099 | hEF1a/TurboGFP inducible PLK4-shRNA | Clone 1; ED Fig 7d–f | This study |
| ODCL0109 | ODCL0099 | hEF1a/TurboGFP inducible PLK4-shRNA | Clone 2; ED Fig 7d–f | This study |
| ODCL0117 | ODCL0088 | TRIM37+/−/−/− (clone 1); CEP192-shRNA | Clonal | This study |
| ODCL0118 | hTERT RPE-1 | CEP192-mNeonGreen; USP28Δ; | Polyclonal | This study |
| TRE3Gpro-Cas9; U6pro-gRNA-CEP192 | ||||
| ODCL0119 | ODCL0061 | TRIM37A; UBCpro-TRIM37 | Clonal | This study |
| ODCL0121 | ODCL0061 | TRIM37Δ; UBCpro-TRIM37-C18R | Clonal | This study |
pro = Promoter; del = deletion; ins = insertion.